Solution reagent kit sCircle®

for autoimmune diseases

Using Singleron's GEXSCOPE® technology, single cells are partitioned into individual microwells, and mRNAs are captured by uniquely barcoded beads. The mRNA are then reverse transcribed into cDNA, followed by a circularization step introduced by a circle handle. By circularization of cDNA, the V(D)J region was brought closer to the sequencing primer. After two rounds of enrichments, the products will be fragmented and constructed for full length VDJ library for illumina short-read sequencing. High RNA capturing efficiency and V(D)J specificity VDJ recombination is the process by which T cell and B cells randomely assemble different gene segments – known as variable (V), diversity (D) and joining (J) genes – in order to generate unique receptors (known as antigen receptors) that can collectively recognize many different types of molecule. The V(D)J region is one of the most highly diversed region on human genome, contributing to complex human immune responses. Therefore understanding the full length sequences of T and B cell V(D)J regions provided a more precise understanding towards the immune processes. Highlights: - Comprehensive - combine transcriptome profiling with full-length TCR or BCR sequences - High throughput - simultaneously process up to 10,000 cells in a single run - High mapping rate - increase the proportion of cells with full-length V(D)J sequences - High capturing efficiency - take advantage of the robust polyA capture Applications 01 Immune Microenvironment Tumor Infiltrating T and B cells 02 Autoimmune Diseases Identify abnormal T and B cells in Immune System 03 Transplantation Identify rejection risks for transplantation