Rotaviruses are a major cause of acute viral gastroenteritis in young animals and children. The virus is transmitted by the fecal-oral route and the infection results in the destruction of mature small intestinal enterocytes.
RIDX Porcine ROTA Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of Rotavirus antigens in porcine feces. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the Rotavirus antigen exists in the sample, it binds to the gold-conjugated rotavirus antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the Rotavirus antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive two monoclonal antibodies to Rotavirus are used as capture and detector in the kit. RIDX Porcine ROTA Ag Test Kit can detect Rotavirus antigens in porcine feces with high accuracy.
Components
• Porcine ROTA Ag test device (10 tests)
• Sample dilution buffer (10 vials)
• Disposable swab (10 ea)
• Disposable dropper (10 ea)
Packages
• 10 Tests/Kit
• 12 Kits/Box (120 Tests/Box) or 40 Kits/Box (400 Tests/Box)
TEST PROCEDURE
1. All reagents and samples must be at room temperature (15~30℃/59~86℉) before use.
2. Collect feces samples using a swab.
3. Insert the swab into the sample dilution tube and mix the swab until the sample has been dissolved into the sample dilution buffer (approximately 10 seconds).
4. Remove the swab from the sample dilution buffer.