Reverse transcriptase reagent RNAok™

Taq DNA polymeraseRNase inhibitorfor RT-PCR

The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Application in vitro translation Separation and identification of specific ribonuclease activities - RNAok™ RNase Inhibitor inhibits RNase A activity Total HeLa RNA (1 µg) was incubated with 1U/µl of RNAok™ RNase Inhibitor and 0.4 ng RNase A for 30 min at 37°C. The result shows that RNAok™ RNase Inhibitor can protect RNA against 0.4 ng RNase A.