Reverse transcriptase reagent kit ExcelRT™

for real-time PCRpHvirus

ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. Features Contains all components for reverse transcription High yield Thermostable, up to 50°C Reduced RNase H ribonuclease activity Application Generation of first strand cDNA from total RNA or mRNA. Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C Contents RTase/RI Enzyme Mix 100 μl 5X RT Buffer (DTT/dNTPs) 500 μl Oligo (dT)/Random Primer Mix 100 μl DEPC-Treated H2O 2 x 1 ml RTase/RI Enzyme Mix ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol 5X RT buffer (DTT/dNTPs) 250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT and 2.5 mM dNTPs (each) Oligo (dT)/Random Primer Mix 50 μM oligo (dT)20 and 100 μM random hexamers