DNA polymerase reagent G-HiFi™

Taq DNA polymerasefor DNA analysispH

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences. Features 5’→3’ DNA polymerase activity 3’→5’ exonuclease (proofreading) activity High reaction rate: 7 seconds/kb High fidelity: 70 times higher than Taq polymerase Generates blunt end amplicons Vast elongation capability (up to 40 kb) Thermo-stable for more than 10 hrs at 95°C Elongation capability G-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM5100). Sensitivity G-HiFi™ DNA Polymerase performs higher sensitivity for high GC content templates (GC: 71%) compare to high fidelity DNA Polymerase from Brand A (M: DM2000). Contents G-HiFi™ DNA Polymerase (1 U/μl) 100 μl 5X G-HiFi™ Buffer 1200 μl dNTPs Mix (2 mM each) 600 μl Storage Buffer 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol