DNA polymerase reagent ExcelTaq™

Taq DNA polymerasefor PCRhot start

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases. The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications Features Aptamer-based hot start PCR Reversible enzyme inactivation Omits extra enzyme activation step Convenient for room temperature PCR set-up High yield and specificity of target amplicons Wide range of amplicon length (up to 10 kb) High sensitivity (as low as 1 fg of plasmid) Applications High specificity PCR High-throughput PCR Generation of PCR products for TA cloning Routine PCR, multiplex PCR, colony PCR, and RT-PCR Hot Start ExcelTaq™ Hot Start II shows absolute amplicon when pre-incubation at 37˚C and 42˚C. Reactions were subjected to a pre-incubation at 37˚C or 42˚C for 10 minutes before initial denaturation step.