The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity (Fig. 1) and high signal intensity when compared with Brand A (Fig. 2). The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Features
High sensitivity and signal intensity
With ROX reference dye
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
High sensitivity
The amplification plot of real-time PCR with cDNA templates ranging from 1.49 fg to 25 ng in quantity, analyzed by using TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) for qPCR amplification.
Better fluorescent signal
SMOBIO’s TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) shows better fluorescent signal as compared to a similar product (Brand A).