FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr.
FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.
Features:
Excellent for in-gel staining
Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)
A safer alternative to EtBr
Compatibility: suitable to blue or UV light
Sensitivity of FluoroVue™
The FluoroVue™ Nucleic Acid Gel Stain (NS1000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment.
Excitation and emission spectrum of FluoroVue™
FluoroVue™ Nucleic Acid Gel Stain (NS1000) has a fluorescent excitation maxima of ~250 and ~482 nm, and an emission maximum of ~509 nm. Therefore, it can replace EtBr without the need for changing existing lab imaging systems.