Loading buffer reagent ExcelBand™

protein ladderfor microbiologyfor molecular biology

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Features Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil. Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue) Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Quality Control Under suggested conditions, PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range resolves 13 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. Specification Product Size 2 x 250 μl MW Range 10 – 180 kDa Band Number 13 Band Color Red/Green/Blue Markered Bands 75, 40, 20 kDa