DNA ladder FluoroBand™

enzymefor DNA extractionpH

The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. Features Sharp bands Quick reference— enhanced bands Ready-to-use— premixed with loading dye for direct loading Stable— room temperature storage over 6 months Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye Source Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range 250 ~ 25,000 bp Concentration 51.6 µg/ 500 µl Recommended loading volume 5 µl/ well Specification Product Size 500 μl Size Range 250 – 25000 bp Band Number 14 Tracking Dye Bromophenol blue and Xylene cyanol FF Enhanced Band 1000 and 3000 bp