Solution reagent SolisFAST® Master Mix

DNA polymeraseTaq DNA polymerasedye

PCR results in 20 minutes reliable results in single and multiplex reactions stable for 2 months at room temperature robust performance suitable Sanger sequencing applications Description SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. Mix Properties Concentration: 5x Hot-start: yes,  2 minutes initial incubation at 98 °C Ready-to-load: no  Cloning Type: T/A cloning Amplicon Size: up to 5 kb Targets: 1-18 targets, depending on target length Mix Components: SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP) and additives that maximize efficiency of PCR Storage The mix can be stored at +4 °C for up to 1 year Stability at room temperature (15 − 25 °C) for 2 months Freeze-thaw stability: 30 cycles Shipping At room temperature