Solution reagent SolisFAST® Master Mix, Ready to Load

buffer solutionDNA polymeraseTaq DNA polymerase

Extremely fast end-point PCR mix for robust and accurate target detection for single- and multiplex reactions PCR Results in 20 minutes analyze one or multiple targets per reaction load directly on gel after PCR Description SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. The mix can be loaded directly to gel for post PCR analysis. Our SolisFAST® Master Mix works the fastest with Eppendorf Mastercycler X50s and Eppendorf Fast PCR Tube Strips. Find out more HERE.* * The cooperation of Solis BioDyne and Eppendorf is limited to SolisFAST® product range and Eppendorf Mastercycler X50 in selected regions: China, Asia Pacific, Africa, Middle East. Mix Properties Concentration: 5x Hot-start: yes,  2 minutes initial incubation at 98 °C Ready-to-load: yes, includes two loading dyes Cloning Type: T/A cloning Amplicon Size: up to 5 kb Targets: 1-18 targets, depending on target length Mix Components: SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP),  additives that maximize efficiency of PCR