Solution reagent SolisFAST® Master Mix, Ready to Load

DNA polymerasebuffer solutiondye

Fast single- and multiplex endpoint PCR assays! PCR Results in 20 minutes. Analyze one or multiple targets per reaction. Load directly on gel after PCR. Description SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. The mix can be loaded directly to gel for post-PCR analysis. Our SolisFAST® Master Mix works the fastest with Eppendorf Mastercycler X50s and Eppendorf Fast PCR Tube Strips. * The cooperation of Solis BioDyne and Eppendorf is limited to SolisFAST® product range and Eppendorf Mastercycler X50 in selected regions: China, Asia Pacific, Africa, Middle East. Mix Properties Concentration: 5x Hot-start: yes, 2 minutes initial incubation at 98 °C Ready-to-load: yes, includes two loading dyes Cloning Type: T/A cloning Amplicon Size: up to 5 kb Targets: 1-18 targets, depending on target length Mix Components: SolisFAST® DNA Polymerase: In silico designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2, dNTPs, additives that maximize efficiency of PCR. Green loading dye Some applications of this product may require a license which is not provided by the purchase of this product.