Enzyme reagent SolisFAST® Master Mix with UNG

solutionDNA polymerasedye

Fast single- and multiplex endpoint PCR assays! PCR Results in 20 minutes. Analyze one or multiple targets per reaction. Contains dUTP and UNG to prevent carryover contamination. Reliable results in Sanger sequencing applications. Description SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). For extra convenience, the mix includes dUTP, and UNG enzyme that enable UNG treatment to prevent carryover contamination. Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping, and reaction set-up. Mix Properties Concentration: 5x Hot-start: yes, 2 minutes initial incubation at 98 °C Ready-to-load: no Cloning Type: T/A cloning Amplicon Size: up to 5 kb Targets: 1-18 targets, depending on target length Mix Components: SolisFAST® DNA Polymerase: In silico designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. PCR Buffer: Includes 17.5 mM MgCl2, dNTPs, UNG, and additives that maximize the efficiency of PCR. Some applications of this product may require a license which is not provided by the purchase of this product.