Enzyme reagent SOLIScript® Fast 1-step RT-qPCR Mix with UNG

DNA polymerasereverse transcriptasedye

4x concentrated one-step RT-qPCR reagent for RNA detection Convenient reaction setup with a single vial format Amplify up to 5 targets within one reaction UNG enzyme prevents carryover contamination and false positive results Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST® DNA Polymerase Robust performance in inhibited conditions RNase inhibitor included in the mix SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results. Applications Detection and quantification of RNA and DNA targets* DNA/LNA hydrolysis probe-based assays Suitable for singleplex and multiplex reactions (up to 5 plex) Properties Sample type: RNA and DNA Product format: Reagent kit (nuclease-free water supplied in a separate tube) Detection type: Probe-based Reference dye: none Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run.