Enzyme reagent SOLIScript® Fast 1-step RT-qPCR Mix with UNG

DNA polymerasereverse transcriptasedye

Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments. 4x concentrated one-step RT-qPCR reagent for RNA detection. Convenient reaction setup with a single vial format. Amplify up to 5 targets within one reaction. UNG enzyme prevents carryover contamination and false positive results. Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST® DNA Polymerase. Robust performance in inhibited conditions. RNase inhibitor included in the mix. Description SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results. Properties Sample type: RNA and DNA Product format: Reagent kit (nuclease-free water supplied in a separate tube) Detection type: Probe-based Reference dye: none* Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run. *Version with ROX available upon request.