Solution reagent SolisFAST® Probe qPCR Mix

DNA polymeraseTaq DNA polymerasefor microbiology

Extremely fast and highly sensitive probe-based qPCR Mix that has been optimized for detection and quantification of up to five targets simultaneously delivers qPCR results 2x faster (in as little as 30 minutes) reliable quantification in up to 5-plex assays consistent results with low- and high-copy targets robust performance stable for 6 months at room temperature tolerant to common PCR inhibitors Description SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in silico- designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice free shipping and reaction set-up. Applications DNA/LNA hydrolysis probe based assays Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination Properties Concentration: 5x Hot-start: yes, 3 minutes initial incubation at 95 °C Cycling speed: fast or ultra-fast Detection type: Probe-based Reference dye: none Multiplex: up to 5 plexes simultaneously Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.