Enzyme reagent Salini UNG® Uracil-N-Glycosylase

solutionfor microbiologymulti-function

Fast and robust heat-labile Uracil-N-Glycosylase Stable at 25 °C for at least 3 months and at 37 °C 2 weeks Heat-labile enzyme (compatible with Sanger sequencing). No reactivation is detected after heat inactivation. Fast 30 second reaction time Tolerant to common inhibitors Reaction set-up and shipment without ice Glycerol-free formulation is available Description Salini UNG® Uracil-N-Glycosylase is a unique heat-labile enzyme. The protein sequence originates from the bacteria genus Salinivibrio which is frequently found in hypersaline environments. Uracil-N-Glycosylase (UNG) efficiently eliminates uracil from single- or doublestranded DNA by catalyzing the hydrolysis of the N-glycosylic bond and leaving an abasic site. This property is widely used as a part of PCR carryover contamination prevention strategy. Salini UNG® is a genetically modified enzyme including a Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that makes all our proteins extremely stable at room temperature [1]. Properties Concentration: 1 U/µl Source: Purified from an E.coli strain that carries an overproducing plasmid containing a Salini UNG® Uracil-N-Glycosylase gene. Storage and dilution buffer: 50% glycerol (v/v), 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers. Recommendations for use Working concentration: For qPCR application we recommend to use Salini UNG® Uracil-N-Glycosylase at a final concentration of 0.025-0.04 U/μl, for endpoint PCR application at a final concentration of 0.005-0.01 U/μl.