Solution reagent Salini UNG® Uracil-N-Glycosylase
enzymefor microbiologyfor research

Solution reagent - Salini UNG® Uracil-N-Glycosylase - Solis BioDyne - enzyme / for microbiology / for research
Solution reagent - Salini UNG® Uracil-N-Glycosylase - Solis BioDyne - enzyme / for microbiology / for research
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Characteristics

Type
solution, enzyme
Applications
for microbiology, for research, laboratory, for PCR, for genotyping, for mutation detection, for drug discovery, industrial, for DNA amplification, for environmental analysis, multi-function, for general purposes
Format
liquid
Storage temperature

-20 °C
(-4 °F)

Description

Fast and robust heat-labile Uracil-N-Glycosylase Stable at 25 °C for at least 3 months and at 37 °C 2 weeks Heat-labile enzyme (compatible with Sanger sequencing). No reactivation is detected after heat inactivation. Fast 30 second reaction time Tolerant to common inhibitors Reaction set-up and shipment without ice Glycerol-free formulation is available Description Salini UNG® Uracil-N-Glycosylase is a unique heat-labile enzyme. The protein sequence originates from the bacteria genus Salinivibrio which is frequently found in hypersaline environments. Uracil-N-Glycosylase (UNG) efficiently eliminates uracil from single- or doublestranded DNA by catalyzing the hydrolysis of the N-glycosylic bond and leaving an abasic site. This property is widely used as a part of PCR carryover contamination prevention strategy. Salini UNG® is a genetically modified enzyme including a Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that makes all our proteins extremely stable at room temperature [1]. Properties Concentration: 1 U/µl Source: Purified from an E.coli strain that carries an overproducing plasmid containing a Salini UNG® Uracil-N-Glycosylase gene. Storage and dilution buffer: 50% glycerol (v/v), 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers. Recommendations for use Working concentration: For qPCR application we recommend to use Salini UNG® Uracil-N-Glycosylase at a final concentration of 0.025-0.04 U/μl, for endpoint PCR application at a final concentration of 0.005-0.01 U/μl.
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