Solution reagent FIREPol®

DNA polymeraseTaq DNA polymerasedye

Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results. Robust amplification in routine applications. Suitable for templates up to 5 kb. Reaction set-up and shipment without ice. TA cloning. Description FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month. Recommended for routine applications (fragment up to 3 kb from genomic DNA). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5). Properties Concentration: 5 U/µl Error rate per nucleotide per cycle is app. 2.5 x 10-5 Accuracy is app. 4 x 104 Estimated half life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Kit Components FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B FIREPol® 10x Buffer BD 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).