Research reagent FIREPol®

solutionDNA polymerasebuffer solution

Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results robust amplification in routine applications suitable for templates up to 5 kb reaction set-up and shipment without ice TA cloning Description FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month. Recommended for routine applications (fragment up to 3 kb from genomic DNA). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5). Properties Concentration: 5 U/µl Error rate per nucleotide per cycle is app. 2.5 x 10-5 Accuracy is app. 4 x 104 Estimated half life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Kit Components FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.