Solution reagent HOT FIREPol®

DNA polymeraseTaq DNA polymerasefor molecular biology

A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up. increased specificity and sensitivity reduced primer-dimer formation reaction set-up and shipment without ice HOT FIREPol® is a chemically modified FIREPol® DNA Polymerase. HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5). Properties Concentration: 5 U/µl Hot-start: yes, activated by a 15 min incubation step at 95°C Error rate per nucleotide per cycle is app. 2.5 x 10-5 Accuracy is app. 4 x 104 Estimated half-life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.