Solution reagent kit HOT FIREPol® MultiPlex Mix

DNA polymeraseTaq DNA polymerasedye

analyze multiple targets per reaction increased sensitivity and yield prevents primer dimer formation reaction set-up and shipment without dry ice Description HOT FIREPol® MultiPlex Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water) and an additional compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. Generated PCR products are compatible with T/A cloning procedures. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Ready to load: yes Fidelity: 1 x Taq Cloning Type: T/A cloning  Multiplexing: tested with up to 18 targets (depending on amplicon length) Amplicon Size: up to 5 kb Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x Multiplex Buffer with 10 mM MgCl2: 1x PCR solution – 2 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration equivalent to 35-45 bp DNA fragment Compound that increases sample density for direct loading