Solution reagent kit SOLIScript® 1-step Probe

enzymefor amplification reactiondiagnostic

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water. cDNA synthesis up to 60°C for superior specificity. Suitable for singleplex and duplex assays. Reaction set-up and shipment without ice. High specificity and sensitivity. One kit for all cyclers. RNase inhibitor included in kit. Description SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. Properties Sample type: RNA Product format: Reagent kit No of tubes: three tubes Detection type: Probe-based Reference dye: reference dye based on ROX Compatible real-time instruments: The Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler! Reagents provided 5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye. 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor. Water, nuclease free.