Enzyme reagent kit SOLIScript® 1-step Probe

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Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water. cDNA synthesis up to 60°C for superior specificity suitable for singleplex and duplex assays reaction set-up and shipment without ice high specificity and sensitivity one kit for all cyclers RNase inhibitor included in kit Description SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube Applications Detection and quantification of RNA targets with qPCR DNA/LNA hydrolysis probe based assay Suitable for singleplex or duplex reactions Properties Sample type: RNA Product format: Reagent kit  No of tubes: three tubes Detection type: Probe-based Reference dye: reference dye based on ROX Compatible real-time instruments: The  Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Reagents  5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor Water, nuclease free