Solution reagent kit SOLIScript® 1-step Multiplex Probe

enzymelaboratorydiagnostic

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water. cDNA synthesis up to 60°C for superior specificity analyze 1-4 targets in 1 reaction reaction set-up and shipment without dry ice robust amplification of GC-rich targets contains dUTP to prevent cross-contamination when used in combination with UNG RNase inhibitor included in kit Description SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays. Applications Detection and quantification of RNA targets with qPCR DNA/LNA hydrolysis probe based assays Suitable for singleplex and multiplex reactions Properties Sample type: RNA Product format: Reagent kit  Detection type: Probe-based Reference dye: none Compatible real-time instruments: The kit is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.  Reagents 5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2) 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor Water, nuclease free