Solution reagent kit SOLIScript® 1-step Multiplex Probe

enzymelaboratorydiagnostic

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water. cDNA synthesis up to 60°C for superior specificity. Analyze 1-4 targets in 1 reaction. Reaction set-up and shipment without dry ice. Robust amplification of GC-rich targets. Contains dUTP to prevent cross-contamination when used in combination with UNG. RNase inhibitor included in kit. Description SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays. Properties Sample type: RNA Product format: Reagent kit Detection type: Probe-based Reference dye: none (ROX and Purple dye available) Compatible real-time instruments: The kit is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler! Reagents provided 5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2) 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor Water, nuclease free