cDNA synthesis and dye-based qPCR in one reaction
Great PCR efficiency – reliable and easy-to-analyze results
High sensitivity – great when working with low concentrated samples
Excellent fluorescence levels
Convenient reaction set-up at room temperature
Environmentally friendly – worldwide ice-free shipping
Description
SOLIScript® 1-step SolisGreen® Kit 2.0 is specially designed for dye-based real-time one-step RT-PCR (RT-qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during qPCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:
higher fluorescence level
better sensitivity for detecting low template concentrations
great stability for room temperature storage
SOLIScript® 1-step SolisGreen® Kit 2.0 is an optimized ready-to-use kit that comes in 3 tubes (including nuclease free water) and contains all the necessary components, except sample (RNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.
Applications
Gene expression analysis and absolute quantification
Pathogen detection and quantification
Low-copy gene detection
miRNA profiling and quantification
Properties
Sample type: RNA
Product format: Reagent kit (nuclease free water supplied in a separate tube)
Detection type: Dye-based, includes SolisGreen® intercalating dye
Reference dye: Based on ROX