Solution reagent kit SOLIScript®

enzymediagnosticfor research

Reverse transcriptase with high thermostability and superior specificity. Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature. Superior performance with gene-specific primers. Reaction temperatures from 37°C to 60°C. Reaction set-up and shipment at room temperature. NB! Not compatible with random primers. Description SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. It can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity. Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme with buffer Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents SOLIScript® Reverse Transcriptase 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT