Solution reagent kit SOLIScript® RT cDNA synthesis

enzymediagnosticfor research

Superior specificity with gene-specific primers in flexible kit format. Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature. Superior specificity with gene-specific primers. Wide reaction temperature 37°C - 60°C. Reaction set-up and shipment without dry ice. In flexible 6-tube kit format. Description SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. It can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity. Properties: Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme with all components included reagent kit Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents provided: SOLIScript® Reverse Transcriptase RiboGrip RNase Inhibitor (40 U/μl) 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT Oligo (dT) Primer (100 μM) dNTP MIX (20 mM of each) Water, nuclease free