Solution reagent kit SOLIScript® RT cDNA synthesis MIX

enzymediagnosticfor research

Superior specificity with gene-specific primers in convenient mix format. Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature. Superior specificity with gene-specific primers. Wide reaction temperature 37°C - 60°C. Reaction set-up and shipment without dry ice. In convenient 3-tube kit format. Description SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. It can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity. Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme mix with all reagents included Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents provided: SOLIScript® Enzyme Mix with SOLIScript® RT and RiboGrip RNase Inhibitor 10x RT Reaction Premix without primers, RT reaction buffer with DTT and dNTPs Water, nuclease free