Fast and sensitive glycerol-free qPCR Mix and a carefully optimized lyophilization excipient mix for effective freeze-drying to cakes or beads.
The kit consists of 2 components: a glycerol-free lyo-compatible qPCR mix and a lyophilization excipient mix
Enables sensitive and specific DNA detection
Contains dUTPs and UNG to prevent carryover contamination
Compatible with fast cycling – results in 30 minutes
Performance preserved after lyophilization
Compatible with lyophilization to beads or cakes
Description
SolisFAST® Lyo-Ready qPCR Kit with UNG is an optimized solution for fast and sensitive amplification and quantitation of DNA targets with probe-based assays. The kit comes in 2 tubes: the glycerol-free 5x SolisFAST® Lyo-compatible qPCR Mix with UNG and 4x SolisFAST® Lyo Excipient Mix. The composition is optimized for lyophilization to cakes or beads.
5x SolisFAST® Lyo-compatible qPCR Mix with UNG is a 5x concentrated qPCR master mix suitable for real-time detection and quantitation of up to five DNA targets simultaneously. It contains dUTPs instead of dTTPs, as well as Salini UNG® Uracil-N-Glycosylase to prevent carry-over contamination from previous amplifications. The mix does not contain any lyophilization additives or excipients.
4x SolisFAST® Lyo Excipient Mix is a 4x concentrated blend of additives that protect reagents during lyophilization. In combination with the glycerol-free qPCR mix, it enables lyophilization to beads or cakes.
Properties
Sample type: DNA
Hot-start: yes, 30 sec to 3 min initial activation at 95 °C
Cycling speed: fast or ultra-fast
Product format: reagent kit
Detection type: probe-based
Reference dye: none