SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is provided in a flexible 5-vial format. Implemented Stability TAG and the unique SoliSD™ Supplement system improves the performance of the enzyme:
Glycerol free format – compatible with lyophilization
Stable for at least 1 month at room temperature
No NTC signal
Fast time to result from 4 minutes
Strong strand displacement activity
Active at 51-62°C with optimum at 60°C
SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C.
SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay. The unique SoliSD™ Supplement system is provided with the kit. Technology improves the performance of the SoliSD™ Bsm DNA Polymerase:
Resolves common NTC signal issue
Minimizes variability between replicates
Allows reaction set-up at room temperature
The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loop-mediated isothermal amplification (LAMP).
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).