DNA polymerase reagent kit 32-23-0000S

for isothermal amplificationLAMP kit

In the new SoliSD™ Lyo-compatible RT-LAMP Kit all enzymes are compatible with freeze- and/or air-drying and contain the patented Stability TAG. The kit is provided in a flexible 5-vial format, with the unique 300X RT-Mix consisting of the blend of Solis BioDyne reverse transcriptases and RNase inhibitor. Glycerol free format – compatible with lyophilization  No NTC signal Strong strand displacement activity of SoliSD™ Bsm Perform RT-LAMP at 55-60°C with optimum at 59°C Description SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C. The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. SoliSD™ Lyo-compatible RT-LAMP Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay. Reagents: SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/μl) 300x RT Mix (FIREScript®; SOLIScript®; RiboGrip®) 10x RT-LAMP Reaction Buffer (200 mM Tris-HCl; 150 mM (NH4)2SO4; 1% Tween-20; 250 mM KCl; additives) 100 mM MgSO4 10x GC-rich Enhancer