Solution reagent kit 01-16-KIT-0000S

DNA polymerasemulti-functionlaboratory

Versatile high-fidelity DNA polymerase for any PCR application requiring precision. High-fidelity amplification – >100x higher than Taq. Superior performance with a variety of amplicons (between 30-79% GC content). Long-range and challenging PCR – up to 24 kb lambda DNA and 12 kb from gDNA. Broad multiplexing capabilities. Efficient dUTP incorporation – great for bisulfite sequencing workflows. Inherently stable due to proprietary Stability TAG technology. Glycerol-free version available upon request. Solis Hot Start HiFi DNA Polymerase is a modified polymerase with 3′→5′ exonuclease (proofreading) activity demonstrating enhanced fidelity of more than 100x compared to wild type Taq polymerase, measured using single-molecule real-time (SMRT) sequencing. Chemical modification of the polymerase enables hot-start PCR that improves specificity and accuracy, minimizing mispriming and extension from non-specifically annealed primers and primer-dimers. Applications: High-fidelity PCR (sequencing library preparation, cloning, protein expression, site-directed mutagenesis, etc.). Long-range PCR and challenging workflows (up to 12 kb gDNA). Amplification of GC-rich templates. Workflows using uracil containing DNA templates or requiring dUTP incorporation – bisulfite sequencing, damaged DNA (FFPE samples), carry-over prevention in combination with UNG. Applications requiring high multiplex capabilities. Glycerol-free version available upon request for freeze-drying applications.