Solution reagent HOT FIREPol® EvaGreen® qPCR Supermix
DNA polymerasedyeTaq DNA polymerase

Solution reagent - HOT FIREPol® EvaGreen® qPCR Supermix - Solis BioDyne - DNA polymerase / dye / Taq DNA polymerase
Solution reagent - HOT FIREPol® EvaGreen® qPCR Supermix - Solis BioDyne - DNA polymerase / dye / Taq DNA polymerase
Solution reagent - HOT FIREPol® EvaGreen® qPCR Supermix - Solis BioDyne - DNA polymerase / dye / Taq DNA polymerase - image - 2
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Characteristics

Type
solution, DNA polymerase, Taq DNA polymerase, dye
Applications
for DNA analysis, for research, for microbiology, diagnostic, laboratory, multi-function, for nucleic acids, for DNA quantification, for genotyping, for amplification reaction, for the pharmaceutical industry, for environmental analysis, industrial, for general purposes, for DNA amplification
Format
liquid
Other characteristics
hot start
Storage temperature

-20 °C
(-4 °F)

Description

Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions. high sensitivity with low DNA concentrations blue dye to ease pipetting reduced primer dimer formation one qPCR mix for all cyclers (except capillary and high ROX* cyclers) contains dUTP to prevent carryover contamination with UNG treatment HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments. The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms.  The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection. Applications Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination Properties Concentration: 5x Hot-start: yes, initial activation in 12 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye*: based on ROX Compatible real-time instruments: most qPCR cyclers. Check Your cycler! IMPORTANT UPDATE! HOT FIREPol® EvaGreen® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems® StepOne™ or StepOnePlus™

Catalogs

Exhibitions

Meet this supplier at the following exhibition(s):

Medlab Middle East 2025
Medlab Middle East 2025

3-06 Feb 2025 Dubai (United Arab Emirates)

  • More information

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    *Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.