Solution reagent HOT FIREPol® EvaGreen® qPCR Supermix

DNA polymeraseTaq DNA polymerasedye

Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting. Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions. High sensitivity with low DNA concentrations. Blue dye to ease pipetting. Reduced primer dimer formation. One qPCR mix for all cyclers (except capillary and high ROX* cyclers). Contains dUTP to prevent carryover contamination with UNG treatment. Description HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments. The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection. Properties Concentration: 5x Hot-start: yes, initial activation in 12 min Detection type: dye-based Reference dye: based on ROX Compatible real-time instruments: most qPCR cyclers*. * HOT FIREPol® EvaGreen® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems® StepOne™ or StepOnePlus™. Mix Components HOT FIREPol® DNA polymerase; 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2; dNTPs, including dUTP; EvaGreen® dye; internal reference based on ROX dye; GC-enhancer; blue visualisation dye.