Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting
Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.
high sensitivity with low DNA concentrations
blue dye to ease pipetting
reduced primer dimer formation
one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
contains dUTP to prevent carryover contamination with UNG treatment
HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.
The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms.
The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.
Applications
Detection and quantification of DNA and cDNA targets
Profiling gene expression
Microbial detection
Viral load determination
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12 min
Detection type: dye-based, includes EvaGreen® intercalating dye
Reference dye*: based on ROX
Compatible real-time instruments: most qPCR cyclers. Check Your cycler!
IMPORTANT UPDATE! HOT FIREPol® EvaGreen® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems® StepOne™ or StepOnePlus™