Solution reagent kit HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

DNA polymeraseTaq DNA polymerasedye

high sensitivity and specificity excellent efficiency reaction set-up and shipment without dry ice cost-effective solution for a wide range of applications Description Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature.  HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on capillary cyclers. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye: none Compatible real-time instruments: LightCycler 1.x and 2.0 (Roche Applied Science). Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction EvaGreen Dye EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye, and has been shown to be non-mutagenic and non-cytotoxic.