Solution reagent kit HOT FIREPol® EvaGreen® qPCR Mix Plus (without ROX)

DNA polymeraseTaq DNA polymerasedye

Cost-effective dye-based qPCR Mix without passive reference dye ROX. High sensitivity and specificity. Excellent efficiency. Reaction set-up and shipment without dry ice. Cost-effective solution for a wide range of applications. Description Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature. HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that do not require passive reference dye. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye: none Compatible real-time instruments: Cyclers that do not require ROX reference dye. Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Some applications of this product may require a license which is not provided by the purchase of this product.