Solution reagent HOT FIREPol® Probe Universal qPCR Mix

DNA polymerasedyeTaq DNA polymerase

Precisely-optimized real time qPCR master mix for probe-based assays. This master mix has been developed for TaqMan® probes but is also suitable for other hydrolysis probe types. suitable for singleplex and duplex assays high specificity and sensitivity one qPCR mix for all cyclers (except capillary) superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG Benchtop stability for 48 hours for preassembled reactions Description HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5' flap endonuclease activity. The Mix contains internal passive reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment. Applications DNA/LNA hydrolysis probe-based assays Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination Properties Concentration: 5x Hot-start: yes, initial activation in 10 min Detection type: Probe-based Reference dye: reference dye based on ROX Compatible real-time instruments: Most qPCR platforms.