Solution reagent HOT FIREPol® Blend Master Mix

DNA polymeraseTaq DNA polymerasefor research

increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Description HOT FIREPol® Blend Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water). HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase. In addition, the mix also allows the amplification of longer fragments. Generated PCR products are compatible with blunt-end and T/A cloning procedures. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min. Ready to load: no Fidelity: 5 x Taq Cloning Type: T/A cloning and Blunt-end cloning Amplicon Size: up to 5 kb Mix Composition: HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme to enhance fidelity 5x Blend Master Mix Buffer with 7,5 mM MgCl2: 1x PCR solution – 1,5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction