Solution reagent HOT FIREPol® Blend Master Mix Ready to Load

agarose gelDNA polymeraseTaq DNA polymerase

Ready to load hot-start PCR master mix for more demanding PCR assays. Increased yield, sensitivity and specificity. Up to 5x higher fidelity. Suitable for templates up to 5 kb. Reduced primer dimer formation. Load directly on gel after PCR. Description HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water), an additional compound needed for direct loading onto an agarose gel, and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase. In addition, the mix also allows the amplification of longer fragments. Generated PCR products are compatible with blunt-end and T/A cloning procedures. Recommendations We recommend using HOT FIREPol® Blend Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. HOT FIREPol® Blend Master Mix Ready to Load is not recommended for use in applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications. Some applications of this product may require a license which is not provided by the purchase of this product.