Research reagent FIREPol® Master Mix

solutionTaq DNA polymerasefor PCR

suitable for templates up to 5 kb reaction set-up and shipment without dry ice all-in-one master mix format reduces pipetting errors and saves time amplicons are suitable for Sanger sequencing post-PCR Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications. Properties Concentration: 5x Hot-start: no Ready to load: no Fidelity: same as Taq Cloning Type: T/A Cloning Amplicon Size: up to 5 kb Mix Components FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP