Solution reagent FIREPol® Master Mix Ready to Load

agarose gelDNA polymeraseTaq DNA polymerase

Ready to load PCR Master Mix for routine application. Load directly to gel after PCR. Suitable for templates up to 5 kb. Reaction set-up and shipment without dry ice. The all-in-one master mix format reduces pipetting errors and saves time. Description Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications. Properties Concentration: 5x Hot-start: no Ready to load: yes Fidelity: 1x Taq Cloning Type: T/A Cloning Amplicon Sixe: up to 5 kb Mix Components FIREPol® DNA polymerase 5x Reaction Buffer B 7.5 mM MgCl2 1 mM dNTPs of each Blue dye Yellow dye Compound that increases sample density for direct loading Some applications of this product may require a license which is not provided by the purchase of this product.