Solution reagent FIREPol® Master Mix Ready to Load

DNA polymeraseagarose gelTaq DNA polymerase

load directly to gel after PCR suitable for templates up to 5 kb reaction set-up and shipment without dry ice all-in-one master mix format reduces pipetting errors and saves time Description Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications. Properties Concentration: 5x Hot-start: no Ready to load: yes Fidelity: 1x Taq Cloning Type: T/A Cloning Amplicon Sixe: up to 5 kb