Solution reagent FIREPol® Master Mix Ready to Load
agarose gelDNA polymeraseTaq DNA polymerase

solution reagent
solution reagent
solution reagent
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Characteristics

Type
solution, agarose gel, DNA polymerase, Taq DNA polymerase
Applications
for electrophoresis, for research, for microbiology, for PCR, for DNA amplification, for nucleic acids, for DNA analysis, for general purposes, for amplification reaction, for genotyping, diagnostic, laboratory, for environmental analysis, for the pharmaceutical industry, industrial, multi-function
Format
liquid
Storage temperature

-20 °C
(-4 °F)

Description

Ready to load PCR Master Mix for routine application. Load directly to gel after PCR. Suitable for templates up to 5 kb. Reaction set-up and shipment without dry ice. The all-in-one master mix format reduces pipetting errors and saves time. Description Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications. Properties Concentration: 5x Hot-start: no Ready to load: yes Fidelity: 1x Taq Cloning Type: T/A Cloning Amplicon Sixe: up to 5 kb Mix Components FIREPol® DNA polymerase 5x Reaction Buffer B 7.5 mM MgCl2 1 mM dNTPs of each Blue dye Yellow dye Compound that increases sample density for direct loading Some applications of this product may require a license which is not provided by the purchase of this product.

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