Solution reagent HOT FIREPol® EvaGreen® HRM Mix (ROX)

DNA polymerasedyebovine serum albumin

highly sensitive mix for accurate SNP detection sensitive EvaGreen® dye for reliable HRM performance reaction set-up without  ice Description High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations. 5x HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers. Applications High-Resolution Melt (HRM) Properties Concentration: 5x Hot-start: yes, initial activation in 12 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye: none Compatible real-time instruments: Cyclers that do not require ROX reference dye.