Solution reagent HOT FIREPol® EvaGreen® HRM Mix (ROX)
DNA polymeraseTaq DNA polymerasedye

solution reagent
solution reagent
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Characteristics

Type
solution, DNA polymerase, Taq DNA polymerase, dye, bovine serum albumin
Applications
laboratory, for microbiology, for research, for nucleic acids, for DNA amplification, for qPCR, for DNA quantification, for general purposes, for DNA analysis, for environmental analysis, for genotyping, multi-function, industrial, for mutation detection, for amplification reaction, diagnostic, for the pharmaceutical industry
Format
liquid
Other characteristics
hot start
Storage temperature

-20 °C
(-4 °F)

Description

Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis. Highly sensitive mix for accurate SNP detection. Sensitive EvaGreen® dye for reliable HRM performance. Reaction set-up without dry ice. Description High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations. 5x HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers. Properties Concentration: 5x Hot-start: yes, initial activation in 12 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye: based on ROX Compatible real-time instruments: Cyclers that require ROX reference dye (Applied BioSystems). Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start. 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution – 2.5 mM MgCl2. dNTPs: dATP, dCTP, dGTP and dTTP. EvaGreen® dye. Bovine serum albumin (BSA) to enhance qPCR reaction. Some applications of this product may require a license which is not provided by the purchase of this product.

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