Solution reagent HOT FIREPol® EvaGreen® HRM Mix (ROX)

DNA polymeraseTaq DNA polymerasedye

Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis. Highly sensitive mix for accurate SNP detection. Sensitive EvaGreen® dye for reliable HRM performance. Reaction set-up without dry ice. Description High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations. 5x HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers. Properties Concentration: 5x Hot-start: yes, initial activation in 12 min Detection type: dye-based, includes EvaGreen® intercalating dye Reference dye: based on ROX Compatible real-time instruments: Cyclers that require ROX reference dye (Applied BioSystems). Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start. 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution – 2.5 mM MgCl2. dNTPs: dATP, dCTP, dGTP and dTTP. EvaGreen® dye. Bovine serum albumin (BSA) to enhance qPCR reaction. Some applications of this product may require a license which is not provided by the purchase of this product.