DNA polymerase reagent kit TERMIPol®

dilution bufferfor microbiologymulti-function

Thermostable DNA polymerase for highly efficient incorporation of unconventional nucleotides. efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides the assay success rate of 99% in MALDI-TOF robust and reliable reaction set-up and shipment without dry ice TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs). Applications Mass-array Properties Concentration: 5 U/µl Hot-start: no Error rate per nucleotide per cycle is app. 8 x 10-5 Estimated half life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers. Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC. Reagents TERMIPol® DNA Polymerase 10x Reaction buffer C 500 mM Tris-HCl pH 9.5 at 25ºC 100 mM MgCl2