DNA polymerase reagent kit TERMIPol®

dilution bufferdiagnosticfor research

DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides. Thermostable DNA polymerase for highly efficient incorporation of unconventional nucleotides. Efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides. The assay success rate of 99% in MALDI-TOF. Robust and reliable. Reaction set-up and shipment without dry ice. Description TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs). Applications MALDI-TOF mass spectrometry Primer extension Mass-array Properties Concentration: 5 U/µl Hot-start: no Error rate per nucleotide per cycle is app. 8 x 10-5 Estimated half-life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers. Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC. Reagents TERMIPol® DNA Polymerase 10x Reaction buffer C 500 mM Tris-HCl pH 9.5 at 25ºC 100 mM MgCl2 Some applications of this product may require a license which is not provided by the purchase of this product.