DNA polymerase reagent kit HOT TERMIPol®

dilution bufferfor microbiologymulti-function

Hot-start DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides. Thermostable hot-start DNA polymerase for highly efficient incorporation of unconventional nucleotides. Efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides. The assay success rate of 99% in MALDI-TOF. Robust and reliable. Reaction set-up and shipment without dry ice. Description HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperature it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 12-15 minute incubation at 95ºC. This prevents the extension of non-specifically annealed primers. The enzyme contains 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs). Properties Concentration: 5 U/µl Hot-start: yes, activated by 12-15 minutes at 95ºC Error rate per nucleotide per cycle is app. 8 x 10-5 Estimated half-life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers. Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC. Reagents HOT TERMIPol® DNA Polymerase 10x Reaction buffer C: 500 mM Tris-HCl pH 9.5 at 25ºC 100 mM MgCl2 Some applications of this product may require a license which is not provided by the purchase of this product.