Enzyme reagent kit FIREScript® RT cDNA synthesis KIT

solutionRNase inhibitorfor microbiology

Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures high specificity and yield wide reaction temperature from 37°C to 60°C fast 15 min reaction time a flexible 7-vial kit with all reaction components RNase inhibitor and water included reaction set-up and shipment without ice Description FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR). Reagents FIREScript® Reverse Transcriptase (200 U/μl) RiboGrip® RNase inhibitor (220 U/μl) 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT) Oligo (dT) Primer (100 μM) Random Primers (100 μM) dNTP MIX (20 mM of each) Water, nuclease free NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.