Enzyme reagent kit FIREScript® KIT

solutionfor microbiologymulti-function

Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures high specificity and yield wide reaction temperature from 37°C to 60°C fast 15 min reaction time a simple 2-vial kit with enzyme and reaction buffer only reaction set-up and shipment without ice Description FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR). Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme and reaction buffer (separate tubes) Enzyme: FIREScript® Reverse Transcriptase Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene. Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.