Glioma detection kit PC219010101

for MGMT Gene Methylationleadclinical

O6-Methylguanine DNA-Methyltransferase (MGMT) is a highly efficient DNA repair enzyme that can repair the damage to the tumor cell DNA caused by alkylating drugs in chemotherapy. A certain level of MGMT activity in cells may lead to drug resistance. MGMT's repair of alkylating agent damage makes it play a dual role in tumor chemotherapy: on the one hand, it protects normal tissues from alkylating agent damage and secondary cancer. On the other hand, it causes the resistance of tumor tissue to alkylating agent chemotherapeutics. DNA methylation is closely related to the occurrence and development of glioma.The“Guidelines for Detection Technology of Tumor Individualized Therapy” states that patients with methylation of the MGMT gene will be benefit from Temozolomide treatment. DETECTION SIGNIFICANCE · MGMT methylation indicates patients will be sensitive to temozolomide chemotherapy, which is helpful for clinical selection of suitable treatments. · MGMT methylation may suggests a better prognosis. APPLICABLE PEOPLE WHO grade III-IV glioma patients before chemotherapy treatment. FEATURES & ADVANTAGES 1. Accuracy and Reliability: Use pre-load PCR tube to effectively avoid cross-contamination. 2. High Sensitivity: Sensitivity can detect as low as 1% gene methylation status. 4. Great versatility: Validated on the most common qPCR machines with stable results. DETECTION PROCESS 1.Nucleic Acid Extraction 2.Modification 4.Amplification 5.Data Analysis