Tuberculosis detection kit AMP202307019

for researchMycobacterium tuberculosisbiological

Tuberculosis, caused by Mycobacterium tuberculosis infection, is one of the significant communicable diseases that pose a serious threat to human health. Drug-resistant mutations in Mycobacterium tuberculosis can develop resistance against anti-tuberculosis drugs. Drug-resistant tuberculosis has always been a focal and challenging aspect in tuberculosis prevention and control efforts. It not only requires prolonged and costly treatment but also has a low cure rate, posing a severe threat to the health of society and the general population. Therefore, accurate detection of drug-resistant mutations against anti-tuberculosis drugs is crucial for the diagnosis and treatment of tuberculosis. DETECTION METHOD Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a novel technique used for the analysis of biological molecules. It involves the formation of a thin co-crystallized film by irradiating the sample with a laser in the presence of a matrix. The matrix absorbs energy from the laser and transfers it to the biological molecules, causing their ionization. The ionized molecules are then accelerated through a flight tube by an electric field, and their time of flight to the detector is used to accurately determine their molecular weight, allowing for precise identification and separation of biomolecules. DETECTION SIGNIFICANCE Accurate identification of common drug-resistant gene mutation sites in clinically prevalent Mycobacterium tuberculosis strains, covering six anti-tuberculosis drugs (Rifampin, Ethambutol, Fluoroquinolones, Isoniazid, Streptomycin, and Pyrazinamide), can assist in the clinical treatment of tuberculosis.