Research detection kit AM202308025

Candida albicansCandida glabrataCandida krusei

With the widespread use of hematopoietic stem cell transplantation, solid organ transplantation, immunosuppressive agents, chemotherapy drugs, and various invasive procedures, the incidence of invasive fungal infections in the lungs is gradually increasing. Patients with pulmonary fungal infections often lack specific symptoms, and early diagnosis is challenging. The condition is easily masked by underlying diseases, leading to misdiagnosis, missed diagnosis, and delayed treatment, resulting in a high mortality rate. Therefore, accurate detection of invasive fungal infections in lung tissue is crucial for the diagnosis and treatment of the disease. DETECTION METHOD Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a novel technique used for the analysis of biological molecules. It involves the formation of a thin co-crystallized film by irradiating the sample with a laser in the presence of a matrix. The matrix absorbs energy from the laser and transfers it to the biological molecules, causing their ionization. The ionized molecules are then accelerated through a flight tube by an electric field, and their time of flight to the detector is used to accurately determine their molecular weight, allowing for precise identification and separation of biomolecules. DETECTION SIGNIFICANCE Accurate identification of the 30 common invasive fungal species in clinical settings provides a clear understanding of the pathogenic organisms and offers a scientific basis for anti-infective treatment.