Pneumococci are enclosed within a capsule consisting of repeated units of polysaccharides. The composition of the polysaccharides defines the serotype, and 91 pneumococcal serotypes have been described to date, with more on the way.
Product description
The polyclonal pool antisera are produced by immunising rabbits with killed whole cells. All the products are absorbed free of cross-reactions. The Pneumotest-kit is supplied in a box with ready-to-use antisera, sufficient for approximately 300 tests.
Many years of experience producing Pneumococcus antisera
High quality
All absorbed free of cross-reactions
We offer experienced technical support
How to use
Pool antisera are used in the capsular reaction, also called the Neufeld test. Equal quantities of antiserum and bacterial culture are mixed on a slide. Under a phase control microscope, a positive reaction is indicated when the capsule enclosing the pneumococcus swells and becomes visible. The bacteria will frequently also agglutinate.
Product range
The Pneumotest-kit consists of 12 pools (A-F + H, P-T).
By testing all 12 pools and using the chessboard system described, it is possible to identify the 23 vaccine serotypes to type/group level. Factor antisera are then used if full serotyping is required.
F.A.Q.
Can I freeze the antibodies?
Antibodies can be frozen, but the process of freezing and thawing is damaging for the antibodies, and the titer will drop (even though not dramatically) due to decomposition.
We have not made further studies on this, and cannot guarantee the stability of the sera, but we have experienced that mostly the titer stays at the same level if a serum only is frozen and thawn once.