Antiserum reagent 54 series

polyclonal antibodyantigenfor bacterial cultures

The H antigen is a surface antigen in E. coli and is responsible for the bacterium’s motility. It also behaves like adhesin. Even if a particular bacterium has genomic material to express H antigens, H antigens are generally expressed only if necessary. At the same time, the quantity of H antigens can vary. It is therefore important that only those strains with known motility are H serotyped, and that they are provoked to express as much flagella as possible. Product description H antisera are polyclonal antibodies produced by immunising rabbits with whole formalin-killed cells containing as much flagellum antigen as possible. All H antisera are absorbed for known cross-reactions and are therefore characterized as monospecific. Cross-reactions with O antigens can occur if the reaction is read after the recommended two hours. A list of known cross-reactions can be provided on request. Pooled H antisera are supplied in bottles of 3 mL ready-to-use antiserum. Individual H antisera are supplied in bottles of 5 mL ready-to-use antiserum. We offer the full range of antisera for E. coli serotyping High quality All absorbed free of cross-reactions We offer experienced technical How to use H antisera are used for agglutination of formalin-killed cultures in Widal tubes or microtitre plates. Equal quantities of antiserum and culture are mixed, and the reaction can be read after approximately 2 hours in a 56 °C water bath. SSI Diagnostica sells H antisera against all 53 defined H serotypes. H antisera are available both as pools (A-F) and as individual antisera, H1-H56 (H13, H22 and H50 have been withdrawn from the H typing classification).