Most E. coli are enclosed in a capsule consisting of polysaccharides, also known as acid polysaccharides. The capsules can be variously shaped and be more or less heat stable.
E. coli K antisera consist of polyclonal antibodies produced in rabbits. The antisera are used for serotyping of the 67 known E. coli K antigens.
Product description
K antisera are polyclonal antibodies produced by immunising rabbits with whole formalin-killed cells expressing as much capsular polysaccharide as possible.
K antisera are supplied ready for use in 1 mL bottles, which is sufficient for at least 65 tests (50 tests for K9 antisera for slide agglutination).
We offer the full range of antisera for E. coli serotyping
High quality
All absorbed free of cross-reactions
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How to use
K antisera are used for current counter immunoelectrophoresis and cannot be used for slide agglutination.
The only exception is K9 antisera, article number 84888, which can be used for slide agglutination for the EPEC strain O104:K9. K9 antisera for slide agglutination was developed during the O104:K9 outbreak in Germany in 2011, and is not for general serotyping of K9 capsules.
Product range
SSI Diagnostica has K antisera against all defined K antigens. The number of K antigens ranges from K2 to K103, but 32 numbers in the sequence are missing. This is because antigens, previously thought to be K antigens, have since then turned out not to be E. coli capsule antigens after all, but Klebsiella and E. coli fimbriae antigens, amongst others. The K antigens eventually proved not to be E. coli capsule antigens are K21, K58-K73, K75-K81, K86, K88, K90, K91, and K99.