Antibody 51172

rabbit serumantigenantiserum

Fimbrial adhesins F4 and F5 (F antigens) help enterotoxigenic E. coli (ETEC) to bind to the intestinal mucosa. By this means they can severely disrupt intestinal function and cause disease in the form of diarrhoea in infants and newborn piglets Product description F antisera are polyclonal antisera produced by immunising rabbits with formalin-killed cells that express as many fimbriae as possible. Previously F4 and F5 were named K88 and K99 and it was wrongly thought that they were capsule antigens. Today, K88 and K99 do no longer exist. F sera are made specific by absorption. SSI Diagnostica supplies F4 and F5 antisera in bottles of 5 mL ready-to-use antiserum, sufficient for at least 250 reactions. We offer the full range of antisera for E. coli serotyping High quality All absorbed free of cross-reactions We offer experienced technical support How to use F antisera are used for slide agglutination with living cultures, and the reaction can be read after 5-10 seconds. Product range F.A.Q. Can I freeze the antibodies? Antibodies can be frozen, but the process of freezing and thawing is damaging the antibodies, and the titer will drop (even though not dramatically) due to decomposition. We have not made further studies on this, and cannot guarantee the stability of the antisera, but we have experienced that the titer mostly stays at the same level if an antiserum is only frozen and thawn once. F4 and F5 antisera are the only antisera in SSI Diagnostica’s product range. My antiserum is outdated. Can I still use it? SSI Diagnostica cannot guarantee that the titer of our antiserum is at the correct level when it has expired.