Solution reagent COAT

buffer solutionfor ELISA

The solution is ready for dilution to specific application requirements and can be used in most ELISA systems. ELISA Plate Coating Solution-10X Concentrate maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding. ELISA Coating Buffer When To Use an ELISA Coating Buffer The primary use for ELISA coating buffers is in immunoassay testing protocols. In a sandwich assay, for example, coating buffers help to immobilize the antibody or antigen coated on the plate. Surmodics™ IVD’s ELISA Coating Buffer (COAT) maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding. When looking to improve assay sensitivity, stability and specificity, assay developers may utilize diluents, blockers, wash buffers, and other ELISA buffers to improve the assay signal, while reducing background noise. Frequently Asked Questions What Is an ELISA Coating Buffer? Surmodics’ BioFX™ ELISA Plate Coating Solution‐10X Concentrate is a physiological saline solution containing a proprietary blend of monobasic and dibasic phosphates. The solution is ready for dilution to specific application requirements and can be used in most ELISA systems. ELISA Plate Coating Solution‐10X Concentrate maximizes specific binding to the target solid phase by providing optimal pH and ionic strength for antigen or antibody binding. How Are ELISA Coating Buffers Made? When assay developers perform preparation protocols for ELISA coating buffers, they must combine the ELISA plate coating solution with distilled water to create a stable solution.